Oxidation of Rosmarinic Acid Catalyzed by Mushroom Tyrosinase
نویسندگان
چکیده
Rosmarinic acid isolated from the Perilla frutescens (L.) was found to possess mushroom tyrosinase (EC 1.14.18.1) inhibitory activities with an IC50 of 4.0 μM. However, rosmarinic acid was oxidized as a substrate catalyzed by tyrosinase. The oxidation kinetics of mushroom tyrosinase by rosmarinic acid measured by spectrophotometic and polarographic methods showed Km=166.8 μM, 225.1 μM and Vmax=16.26 μM/min, 3.05 mg O2/L/min, respectively.
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